Enhancement of growth and cellulose accumulation by overexpression of xyloglucanase in poplar

FEBS Letters

Hayashi, Takahisa⁴  Iida, Ikuho²  Arai, Motoh¹  Park, Yong Woo⁴  Sameshima, Kazuhiko³  Baba, Kei'ichi⁴  Furuta, Yuzo² 

[1] Department of Applied Biochemistry, Osaka Prefectural University, 1-1 Gakuen-cho, Sakai 599-8531, Japan;Biological Function Science Course, Kyoto Prefectural University, Shimogamo-nakaragi-cho, Sakyo-ku, Kyoto 606-8522, Japan;Department of Forest Science, Kochi University, Nangoku 783-8502, Japan;Wood Research Institute, Kyoto University, Gokasho, Uji 611-0011, Japan

关键词: Cellulose deposition;    Secondary wall;    Transgenic poplar;    Xyloglucan;    Xyloglucanase;   

DOI  :  10.1016/S0014-5793(04)00346-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Because the loosening of xyloglucan in the cell wall promotes plant growth (Takeda et al. (2002) Proc. Natl. Acad. Sci. USA 99, 9055–9060; Park et al. (2003) Plant J. 33, 1099–1106), we expressed Aspergillus xyloglucanase constitutively in Populus alba. The expression increased the length of stem even in the presence of sucrose. Increased stem growth was accompanied by a decrease in Young's elastic modulus in the growth zone but an increased elasticity in mature tissue. The increased internode length corresponded to an increase in cellulose content as well as specific gravity, showing that the removal of xyloglucan might cause an increase in cellulose density in the secondary xylem.

【 授权许可】

Unknown