Disruption of type 5 adenylyl cyclase negates the developmental increase in Gαolf expression in the striatum

FEBS Letters

Okumura, Satoshi³  Toya, Yoshiyuki¹  Hashimoto, Yoko¹  Tsunematsu, Takashi¹  Iwatsubo, Kousaku¹  Hervé, Denis²  Ishikawa, Yoshihiro¹  Iwamoto, Tamio¹  Umemura, Satoshi¹ 

[1] Departments of Physiology and Medicine, Yokohama City University School of Medicine, Yokohama 234-0002, Japan;INSERM/UPMC U536, Institut du Fer à Moulin, 75005 Paris, France;Cardiovascular Research Institute, Department of Cell Biology and Molecular Medicine and Medicine (Cardiology), New Jersey Medical School, University of Medicine and Dentistry of New Jersey, Newark, NJ 07103, USA

关键词: Adenylyl cyclase;    Golf;    Gs;    Knockout;    Striatum;    Development;   

DOI  :  10.1016/S0014-5793(04)00333-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The two stimulatory G protein α subunits, Gαs and Gαolf, activate adenylyl cyclase in a similar way. We examined whether type 5 adenylyl cyclase knockout, the major striatal isoform, can differentially and/or developmentally change the expression of these G proteins in the striatum. Gαs and Gαolf expressions at birth were unaffected in knockouts, which, however, demonstrated a blunted developmental increase in Gαolf, but not Gαs. Adenylyl cyclase activity was unaffected at birth, but subsequently became lower in knockouts. These findings suggest that type 5 adenylyl cyclase does not contribute to striatal cAMP signaling at birth. However, it may play an important role in developmental changes in the expression of Gαolf, but not Gαs.

【 授权许可】

Unknown