Interaction of actin and its 11‐amino acid C‐terminal peptide as cofactors with the adenovirus proteinase

FEBS Letters

Mangel, Walter F²  Brown, Mark T¹ 

[1] Department of Pharmacological Sciences, State University of New York at Stony Brook, Stony Brook, NY 11794, USA;Biology Department, Brookhaven National Laboratory, Upton, NY 11973, USA

关键词: Cysteine proteinase;    Viral proteinase;    Cell lysis;    Equilibrium dissociation constant;    Cytoskeleton destruction;    Enzyme activation;    11-Actin-C;    the 11-amino acid residue peptide from the C-terminus of actin (SGPSIVHRKCF);    AVP;    adenovirus proteinase;    AVP–pVIc;    covalent complex between AVP and pVIc formed by the disulfide bond between Cys104 of AVP and Cys10′ of pVIc;    AVP(pVIc);    reversible complex between AVP and pVIc;    AVP(actin);    reversible complex between AVP and actin;    AVP(DNA);    reversible complex between AVP and DNA;    k cat;    catalytic rate constant for substrate hydrolysis;    K d;    equilibrium dissociation constant;    K m;    Michaelis constant;    pVIc;    11-amino acid peptide (GVQSLKRRRCF) that originates from the C-terminus of the viral precursor protein pVI;    Random-Sequence-Peptide;    an 11-amino acid peptide with the same amino acids as in pVIc but in a sequence that was generated randomly (SFRRCGLRQVK);   

DOI  :  10.1016/S0014-5793(04)00285-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Actin bound to the adenovirus proteinase (AVP) with a lower equilibrium dissociation constant, 4.2 nM, than those exhibited by two viral, nuclear cofactors for AVP, the 11-amino acid peptide pVIc and the viral DNA. The k _cat/K _m ratio for substrate hydrolysis by AVP increased 150 000-fold in the presence of actin. The 11-amino acid residue peptide corresponding to the C-terminus of actin, which is highly homologous to pVIc, bound to AVP and stimulated its activity in the presence of DNA. As a cellular cofactor for AVP, AVP(actin) complexes may facilitate the cleavage of cytoskeletal proteins, preparing the infected cell for lysis and release of nascent virions.

【 授权许可】

Unknown