Up‐regulation of Na+,K+‐ATPase α3‐isoform and down‐regulation of the α1‐isoform in human colorectal cancer

FEBS Letters

Horikawa, Naoki²  Maeda, Mizuki¹  Takahashi, Yuji¹  Minamimura, Tetsuji²  Takeguchi, Noriaki¹  Suzuki, Tomoyuki¹  Sakai, Hideki¹  Tsukada, Kazuhiro² 

[1] Department of Pharmaceutical Physiology, Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, 2630 Sugitani, Toyama 930-0194, Japan;Department of Surgery II, Faculty of Medicine, Toyama Medical and Pharmaceutical University, 2630 Sugitani, Toyama 930-0194, Japan

关键词: Na+;    K+-ATPase;    α-Isoform;    Colon;    Cancer;   

DOI  :  10.1016/S0014-5793(04)00292-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We investigated expression levels of Na⁺,K⁺-ATPase α-isoforms and their ATPase activities in human colorectal cancer tissue and the accompanying normal mucosa. A decrease in expression of the α1-isoform protein was observed in all sampled cancer tissues compared with the normal mucosae. The level of ouabain (5 μM)-sensitive Na⁺,K⁺-ATPase activity in carcinomas was 81±5% that of in the normal mucosae. The mRNA expression of α2- and α4-isoforms was decreased in almost all the carcinoma samples. Interestingly, the expression level of the α3-isoform protein in the cancer tissue was higher than that of the normal mucosa. These results indicate that a decrease in the α1-isoform expression and an increase in the α3-isoform expression may be associated with colorectal cancer.

【 授权许可】

Unknown