The membrane topology of EmrE – a small multidrug transporter from Escherichia coli

FEBS Letters

Schuldiner, Shimon¹  Ninio, Shira¹  Elbaz, Yael¹ 

[1] Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, 91904 Jerusalem, Israel

关键词: EmrE;    Membrane protein;    Drug resistance;    Smr;    Cysteine accessibility;   

DOI  :  10.1016/S0014-5793(04)00240-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

EmrE is a multidrug transporter from Escherichia coli that belongs to the Smr family of small multidrug transporters. The secondary structure of EmrE consists of a four helical bundle, as judged by different techniques. EmrE has been extensively characterized; nevertheless, the membrane topology of EmrE has not been determined yet. Previous work with a homologous Smr protein provided partial information of the membrane topology, however the location of the carboxy-terminus remained inconclusive. In this work we probed the membrane topology of EmrE, focusing on the carboxy-terminus of the protein, using two independent approaches. Our results support a secondary structure where the carboxy-terminus faces the cytoplasm, while the first loop faces the periplasm.

【 授权许可】

Unknown