Applied jasmonates accumulate extracellularly in tomato, but intracellularly in barley

FEBS Letters

Stenzel, Irene¹  Hause, Bettina¹  Bücking, Heike²  Förster, Hella¹  Miersch, Otto¹ 

[1]Institute of Plant Biochemistry, Weinberg 3, D-06120 Halle, Germany

[2]Department of Plant Biology, Michigan State University, East Lansing, MI 48824-1312, USA

关键词: 14C-labeled jasmonic acid;    Barley;    In situ hybridization;    Microautoradiography;    Tomato;    AOC;    allene oxide cyclase;    AOS;    allene oxide synthase;    DIG;    digoxigenin;    JA;    jasmonic acid;    JIP;    jasmonate-induced protein;    OPDA;    12-oxo-phytodienoic acid;    PBS;    phosphate-buffered saline;    PIN;    proteinase inhibitor;   

DOI  :  10.1016/S0014-5793(04)00178-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Jasmonic acid (JA) and its derivatives are well-characterized signaling molecules in plant defense and development, but the site of their localization within plant tissue is entirely unknown. To address the question whether applied JA accumulates extracellularly or intracellularly, leaves of tomato and barley were fed with ¹⁴C-labeled JA and the label was localized in cryofixed and lyophilized leaf tissues by microautoradiography. In tomato the radioactivity was detectable within the apoplast, but no label was found within the mesophyll cells. By contrast, in barley leaf tissues, radioactivity was detected within the mesophyll cells suggesting a cellular uptake of exogenously applied JA. JA, applied to leaves of both plants as in the labeling experiments, led in all leaf cells to the expression of JA-inducible genes indicating that the perception is completed by JA signal transduction.

【 授权许可】

Unknown