Chemical modification of glucose oxidase: possible formation of molten globule‐like intermediate structure

FEBS Letters

Nemat-Gorgani, Mohsen²  Naderi-Manesh, Hossein¹  Ranjbar, Bijan¹  Hosseinkhani, Saman² 

[1] Department of Biochemistry, Faculty of Basic Sciences, Tarbiat Modarres University, P.O. Box 14115-175, Tehran, Iran;Institute of Biochemistry and Biophysics, University of Tehran, P.O. Box 13145-1384, Tehran, Iran

关键词: Chemical modification;    Glucose oxidase;    Molten globule;    8-Anilino-1-naphthalenesulfonic acid;    Intrinsic fluorescence;    Circular dichroism;    ANS;    8-anilino-1-naphthalenesulfonic acid;    CD;    circular dichroism;    FAD;    flavin adenine dinucleotide;   

DOI  :  10.1016/S0014-5793(04)00134-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Chemical modification of lysine residues in glucose oxidase was carried out using citraconic anhydride. Modification brought about changes in the kinetic properties of the enzyme as evident by substantial lowering of V _max and K _m. Enhancement of tryptophan fluorescence was observed with a dramatic change in its pH dependence due to modification. Near- and far-UV circular dichroism spectra of the native and modified forms suggested formation of molten globule-like structures, further supported by 8-anilino-1-naphthalenesulfonic acid fluorescence which indicated higher exposure of hydrophobic residues as a result of chemical modification.

【 授权许可】

Unknown