AtHMA3, a plant P1B‐ATPase, functions as a Cd/Pb transporter in yeast

FEBS Letters

Verret, Frédéric¹  Richaud, Pierre¹  Lieutaud, Aurélie¹  Auroy, Pascaline¹  Vavasseur, Alain¹  Gravot, Antoine¹ 

[1] CEA Cadarache, DSV/DEVM/Laboratoire des Echanges Membranaires et Signalisation, UMR 6191 CNRS-CEA-Aix-Marseille II, Bat. 156, 13108 St Paul lez Durance Cedex, France

关键词: P-type adenosine triphosphatase;    AtHMA3;    Cadmium;    Lead;    Zinc;    Metal tolerance;    Arabidopsis;    GFP;    green fluorescent protein;    Ws;    Wassilewskija;    Col;    Columbia;   

DOI  :  10.1016/S0014-5793(04)00072-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The Arabidopsis thaliana AtHMA3 protein belongs to the P_1B-adenosine triphosphatase (ATPase) transporter family, involved in heavy metal transport. Functional expression of AtHMA3 phenotypically complements the Cd/Pb-hypersensitive yeast strain Δycf1, but not the Zn-hypersensitive mutant Δzrc1. AtHMA3-complemented Δycf1 cells accumulate the same amount of cadmium as YCF1-complemented Δycf1 cells or wild-type cells, suggesting that AtHMA3 carries out an intracellular sequestration of Cd. A mutant of AtHMA3 altered in the P-ATPase phosphorylation domain did not complement Δycf1, suggesting that metal transport rather than chelation is involved. The fusion protein AtHMA3::green fluorescent protein (GFP) is localized at the vacuole, consistent with a role in the influx of cadmium into the vacuolar compartment. In A. thaliana, the mRNA of AtHMA3 was detected mainly in roots, old rosette leaves and cauline leaves. The expression levels were not affected by cadmium or zinc treatments.

【 授权许可】

Unknown