Neurofibromin is actively transported to the nucleus

FEBS Letters

Vandenbroucke, Ina²  Coene, Elisabeth¹  Van Oostveldt, Patrick³  Messiaen, Ludwine²  De Paepe, Anne² 

[1] Department of Pathology, Ghent University Hospital, 9000 Ghent, Belgium;Centre for Medical Genetics, Ghent University Hospital, De Pintelaan 185-0K5, 9000 Ghent, Belgium;Department of Molecular Biotechnology, Ghent University, 9000 Ghent, Belgium

关键词: Neurofibromatosis type 1;    Neurofibromin;    Nuclear localization signal;    Nuclear localization;    Alternative splicing;    NF1;    neurofibromatosis type 1;    GAP;    GTPase-activating protein;    GRD;    Gap-related domain;    NLS;    nuclear localization signal;    GFP;    green fluorescent protein;    FLIP;    fluorescence loss in photobleaching;    ROI;    region of interest;   

DOI  :  10.1016/S0014-5793(04)00078-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Mutations in the neurofibromatosis type 1 (NF1) tumor suppressor gene predispose individuals to a variety of benign and malignant tumors. Many tumor suppressors ‘shuttle’ between the nucleus and the cytoplasm, thus regulating their function. By expressing different NF1 constructs in COS-7 cells (encompassing exons 28–49 and fused to the green fluorescent protein), we identified a functional nuclear localization signal (NLS) in exon 43. Mutation of the NLS completely abolishes the nuclear entry of the NF1-derivative fusion protein. A highly expressed splice variant that lacks this NLS controls the localization and hence the function of neurofibromin. The localization of neurofibromin in the nucleus may provide novel clues to unknown functions for NF1.

【 授权许可】

Unknown