Increased transcription of mitochondrial genes for Complex I in human platelets during ageing

FEBS Letters

Lenaz, Giorgio²  Cocco, Lucio³  Faenza, Irene³  Merlo Pich, Milena²  Raule, Nicola²  Fagioli, Maria Elena¹  Catani, Lucia¹ 

[1] Institute of Hematology and Medical Oncology ‘L. e A. Seràgnoli’, University of Bologna, Via Massarenti 9, Bologna, Italy;Department of Biochemistry ‘G. Moruzzi’, University of Bologna, Via Irnerio 48, 40126 Bologna, Italy;Cellular Signalling Laboratory, Institute of Anatomy, University of Bologna, Via Irnerio 48, 40126 Bologna, Italy

关键词: Mitochondrial mRNA;    Ageing;    Oxidative phosphorylation system;    Gene expression;    mtDNA;    mitochondrial DNA;    ROS;    reactive oxygen species;    OXPHOS;    oxidative phosphorylation;    COX;    cytochrome c oxidase;    ND;    reduced nicotinamide adenine dinucleotide (NADH) dehydrogenase;    GAPDH;    glyceraldehyde-3-phosphate dehydrogenase;   

DOI  :  10.1016/S0014-5793(03)01520-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We studied the effect of ageing on the mRNA levels of mitochondria-encoded polypeptides in human platelets. We used quantitative real-time reverse transcriptase-polymerase chain reaction (RT-PCR) to investigate the expression of selected cytochrome c oxidase (COX) genes (subunits I and III) and Complex I genes (subunits reduced nicotinamide adenine dinucleotide (NADH) dehydrogenase (ND)1 and ND5) in platelets from young and aged healthy subjects. Northern blot analysis confirmed the PCR results. COX I expression is higher than that of COX III in both young and aged platelets. A significant increase of transcripts for Complex I was found during ageing. On the contrary, the mRNA levels of the two COX subunits did not significantly vary during ageing.

【 授权许可】

Unknown