Absence of mechanical allodynia and Aβ‐fiber sprouting after sciatic nerve injury in mice lacking membrane‐type 5 matrix metalloproteinase

FEBS Letters

Okada, Akiko²  Hayashita-Kinoh, Hiromi²  Yana, Ikuo²  Nonaka, Takahiro²  Kinoh, Hiroaki²  Seiki, Motoharu²  Yoshida, Nobuaki¹  Komori, Kiyoshi² 

[1] Division of Gene Expression and Regulation, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokane-dai, Minato-ku, Tokyo 108-8639, Japan;Department of Cancer Cell Research, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokane-dai, Minato-ku, Tokyo 108-8639, Japan

关键词: Matrix metalloproteinase;    Membrane-type matrix metalloproteinase;    Gene targeting;    Mechanical allodynia;    Sprouting;    MMP;    matrix metalloproteinase;    MT-MMP;    membrane-type matrix metalloproteinase;    ECM;    extracellular matrix;    CTB;    cholera toxin B subunit;    ES;    embryonic stem;    CSPG;    chondroitin sulfate proteoglycan;   

DOI  :  10.1016/S0014-5793(03)01458-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Matrix metalloproteinases (MMPs) are a family of endopeptidases that degrade extracellular matrix components. Membrane-type 5 MMP (MT5-MMP/MMP-24) was identified as neuron-specific, and is believed to contribute to neuronal circuit formation and plasticity. To elucidate its function in vivo, we have generated mice lacking MT5-MMP by gene targeting. MT5-MMP-deficient mice were born without obvious morphological abnormalities. No apparent histological defects were observed in the nervous system either. However, MT5-MMP-deficient mice did not develop neuropathic pain with mechanical allodynia after sciatic nerve injury, though responses to acute noxious stimuli were normal. Neuropathic pain induced by peripheral nerve lesions is known to accompany structural reorganization of the nervous system. Intraneural injection of cholera toxin B subunit, a transganglionic tracer, into the injured sciatic nerve of wild-type mice revealed that the myelinated Aβ-fiber primary afferents sprouted from laminae III–VI of the dorsal horn of the spinal cord and invaded lamina II. However, no such sprouting and invasion of Aβ-fibers were observed in MT5-MMP-deficient mice. These findings suggest that MT5-MMP is essential for the development of mechanical allodynia and plays an important role in neuronal plasticity in this mouse model.

【 授权许可】

Unknown