Results and prospects of the yeast three‐hybrid system

FEBS Letters

Martin, Franck¹  Eriani, Gilbert¹  Jaeger, Sophie¹ 

[1] Institut de Biologie Moléculaire et Cellulaire, UPR No. 9002 du CNRS, 15 rue René Descartes, 67084 Strasbourg Cedex, France

关键词: Yeast three-hybrid system;    RNA–protein interactions;    HBP/SLBP;    Histone mRNA;   

DOI  :  10.1016/S0014-5793(03)01434-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In 1996, a new method, termed the yeast three-hybrid system, dedicated to selection of RNA binding proteins using a hybrid RNA molecule as bait was described. In this minireview, we summarize the results that have been obtained using this method. Indeed, ∼20 unknown proteins have been characterized so far. The three-hybrid strategy has also been used as a tool to dissect RNA–protein interactions. The example of such a study on human histone HBP interaction with its target mRNA is described. Problems that can be encountered are addressed in a troubleshooting section. Especially, our results with tRNA binding proteins are discussed.

【 授权许可】

Unknown