Newly identified exons encoding novel variants of p94/calpain 3 are expressed ubiquitously and overlap the α‐glucosidase C gene

FEBS Letters

Hata, Shoji²  Ito, Yoshimasa¹  Abe, Keiko²  Kawabata, Yukiko²  Ono, Yasuko²  Sorimachi, Hiroyuki²  Suzuki, Koichi³ 

[1] School of Pharmaceutical Sciences, Kinki University, Higashi-Osaka 577-8502, Japan;Laboratory of Biological Function, Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan;New Frontiers Research Laboratories, Toray Industries Inc., Kamakura 248-8555, Japan

关键词: Calpain;    Alternative promoter;    Ubiquitous expression;    Proteolytic processing;   

DOI  :  10.1016/S0014-5793(03)01324-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

There are two classes of an intracellular ‘modulator protease’, calpain: ubiquitous and tissue-specific. p94/calpain 3 is an example of the latter, predominantly expressed in muscle. A defect in the p94 gene causes muscular dystrophy. Here we report that human and mouse p94 genes have a possible novel alternative promoter expressing p94 variants in all tissues examined including human lens epithelial cells. The possible promoter region and the following novel exons overlap the 3′ region of the neutral α-glucosidase C gene. Unlike p94, the novel p94 variants expressed in COS7 cells do not undergo rapid autolysis, suggesting basic functions different from p94.

【 授权许可】

Unknown