Growth hormone induces eNOS expression and nitric oxide release in a cultured human endothelial cell line

FEBS Letters

Frölich, Jürgen C¹  Thum, Thomas²  Borlak, Jürgen²  Tsikas, Dimitris¹ 

[1] Institute of Clinical Pharmacology, Hannover Medical School, 30625 Hannover, Germany;Fraunhofer Institute of Toxicology and Experimental Medicine (ITEM), Drug Research and Medical Biotechnology, 30625 Hannover, Germany

关键词: Growth hormone;    Somatotropin;    Endothelial nitric oxide synthetase;    Nitric oxide;    Growth hormone deficiency;    Reactive oxygen species;    Endothelial cell;   

DOI  :  10.1016/S0014-5793(03)01356-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Growth hormone deficiency is linked to cardiovascular disease and particularly increased peripheral vascular resistance. Surprisingly, its role in endothelial nitric oxide (NO) synthetase (eNOS) regulation and NO release is basically unknown. We therefore studied the effects of different doses of somatotropin in cultures of a human endothelial cell line (EAhy926). We investigated expression and activity of eNOS, as well as other target genes known to be deregulated in cardiovascular disease including E-selectin and the lectin-like oxidized low density lipoprotein receptor. Treatment of cultured human endothelial cells with somatotropin resulted in significant (P<0.05) increases of eNOS gene and protein expression, as well as NO release, whereas production of intracellular reactive oxygen species was significantly reduced, at the highest somatotropin dose level. The enhanced eNOS gene/protein expression and enzyme activity correlate well. Our findings are suggestive for a novel role of growth hormone in endothelial biology, and particularly NO production.

【 授权许可】

Unknown