Properties of the co‐chaperone protein p23 erroneously attributed to ALG‐2 (apoptosis‐linked gene 2)

FEBS Letters

Nielsen, Per F¹  Krogh, Thomas N¹  Mollerup, Jens²  Berchtold, Martin W² 

[1] Protein Science, Novo Nordisk A/S, Novo Allé, 2880 Bagsværd, Denmark;Department of Molecular Cell Biology, Institute of Molecular Biology, University of Copenhagen, Øster Farimagsgade 2A, 1353 Copenhagen K, Denmark

关键词: Heterogeneous ribonuclear protein A2/B1;    Prostaglandin E2 synthase;    PYK2;    Ischemia;    Cancer;    Atherosclerosis;    hnRNP;    heterogeneous ribonuclear protein A2/B1;    cPGES;    cytoplasmic prostaglandin E2 synthase;    ALG-2;    apoptosis-linked gene 2;    COX;    cyclooxygenase;    MALDI-TOF;    matrix assisted laser desorption ionisation time of flight;    MS;    mass spectrometry;    PG;    prostaglandin;    HSP90;    heat shock protein 90;   

DOI  :  10.1016/S0014-5793(03)01310-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A commercial antibody (clone 22) directed against the apoptosis-linked gene 2 (alg2, pdcd6) encoded protein has been used by several groups. Up-regulated expression of the antigen was observed in primary tumours and in metastatic tissue and also during rat brain ischemia. Furthermore, antigen down-regulation was found in human atherosclerotic plaques. Recently, we found that the clone 22 antibody does not recognise ALG-2. In the present study the antigen of the clone 22 antibody was identified as the heat shock protein 90 (HSP90) co-chaperone protein p23, identical to the cytosolic prostaglandin E2 synthase, by immunoprecipitation followed by tryptic in-gel digests and mass spectrometry of the purified peptides. Moreover, the heterogeneous ribonuclear protein A2/B1 was found to be a part of the p23 co-immunoprecipitated protein complex.

【 授权许可】

Unknown