Plant mitochondrial nucleoside diphosphate kinase is attached to the membrane through interaction with the adenine nucleotide translocator

FEBS Letters

Knorpp, Carina¹  Baird, Anne-Marie¹  Johansson, Monika¹ 

[1] Department of Plant Biology and Forest Genetics, The Swedish University of Agricultural Sciences, Box 7080, S-750 07 Uppsala, Sweden

关键词: Plant mitochondrion;    Nucleoside diphosphate kinase;    Adenine nucleotide translocator;    ANT;    adenine nucleotide translocator;    IMS;    intermembrane space;    mNDPK;    mitochondrial nucleoside diphosphate kinase;    PMSF;    phenylmethylsulfonyl fluoride;   

DOI  :  10.1016/S0014-5793(03)01288-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

This study shows that the plant mitochondrial nucleoside diphosphate kinase (mNDPK) localizes to both the intermembrane space and to the mitochondrial inner membrane. We show that mNDPK is very firmly attached to the membrane. Co-immunoprecipitation experiments identified the adenine nucleotide translocator as an interaction partner. This is the first report showing a direct association between these two proteins, although previous studies have shown metabolic cooperation between them. Possible consequences for mitochondrial energy metabolism are discussed.

【 授权许可】

Unknown