Inflammatory prompts produce isoform‐specific changes in the expression of leukotriene B4 ω‐hydroxylases in rat liver and kidney

FEBS Letters

Robida, Aaron M²  Antonovic, Leposava¹  Strobel, Henry W¹  Morgan, Edward T²  Kalsotra, Auinash¹  Cui, Xiaoming¹ 

[1] Department of Biochemistry and Molecular Biology, University of Texas Medical School at Houston, Houston, TX 77030, USA;Department of Pharmacology, Emory University, Atlanta, GA 30322, USA

关键词: Inflammation;    Leukotriene B4 ω-hydroxylase;    Cytochrome P450 4F;    Interleukin-1;    Lipopolysaccharide;    Chlorpromazine metabolism;    CYP and P450;    cytochrome P450;    QRTPCR;    quantitative real time polymerase chain reaction;    LTB4;    leukotriene B4;    HETE;    hydroxyeicosatetraenoic acid;    LPS;    lipopolysaccharide;    IL-1;    interleukin-1;    TNF-α;    tumor necrosis factor-α;    CPZ;    chlorpromazine;   

DOI  :  10.1016/S0014-5793(03)01240-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Cytochrome P450 (CYP) 4Fs metabolize leukotriene B₄ and other inflammatory mediators in the arachidonic acid cascade. Here we show that lipopolysaccharide (LPS) treatment suppresses CYP4F4 and up-regulates CYP4F5 mRNA expression in rat liver whereas renal CYP4Fs are essentially unchanged. BaSO₄ treatment, in contrast, increases both hepatic and renal CYP4F expression levels. Thus, distinct regulatory mechanisms in CYP4F expression might operate under different inflammatory prompts. To examine hepatic totipotency, primary hepatocytes were treated with varying doses of LPS resulting in decrease in all the CYP4F isoforms. Treatment of hepatocytes with 5 ng/ml of interleukin-1β mimics the in vivo effects of LPS on CYP4F expression.

【 授权许可】

Unknown