Outer membrane protein A of Escherichia coli forms temperature‐sensitive channels in planar lipid bilayers

FEBS Letters

Zakharian, E¹  Reusch, R.N¹ 

[1] Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI 48824, USA

关键词: Outer membrane protein A;    Channel;    Planar bilayer;    Temperature-sensitive;    Escherichia coli;   

DOI  :  10.1016/S0014-5793(03)01236-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The temperature dependence of single-channel conductance and open probability for outer membrane protein A (OmpA) of Escherichia coli were examined in planar lipid bilayers. OmpA formed two interconvertible conductance states, small channels, 36–140 pS, between 15 and 37°C, and large channels, 115–373 pS, between 21 and 39°C. Increasing temperatures had strong effects on open probabilities and on the ratio of large to small channels, particularly between 22 and 34°C, which effected sharp increases in average conductance. The data infer that OmpA is a flexible temperature-sensitive protein that exists as a small pore structure at lower temperatures, but refolds into a large pore at higher temperatures.

【 授权许可】

Unknown