Ultraviolet radiation‐induced DNA damage in promoter elements inhibits gene expression

FEBS Letters

Tummala, Ramakumar¹  Ghosh, Rita¹  Mitchell, David L² 

[1] Department of Cancer Causation and Prevention, AMC Cancer Research Centre and the University of Colorado Comprehensive Cancer Centre, 1600 Pierce Street, Denver, CO 80214, USA;Department of Carcinogenesis, MD Anderson Cancer Centre, Smithville, TX 78957, USA

关键词: Ultraviolet radiation;    DNA damage;    Gene expression;   

DOI  :  10.1016/S0014-5793(03)01215-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Repair of DNA damage in gene promoters is slower than in actively transcribed genes. Persistent damage in gene promoters though transient can have significant biological effects on regulated gene expression. In this study we investigated the effect of ultraviolet radiation on gene promoter-associated functions when DNA damage is located within and outside transcription factor binding sites. Our results show that both cyclobutane pyrimidine dimers and (6-4) photoproducts inhibit DNA–protein interaction, in vitro transcript production and transactivation of reporter genes. The biological significance of transient DNA damage as a mechanism in carcinogenesis is discussed.

【 授权许可】

Unknown