Metabolic strategy of boar spermatozoa revealed by a metabolomic characterization

FEBS Letters

Chiang, Kelly²  Marin, Silvia²  Boros, Laszlo G¹  Fernández-Novell, Josep Maria²  Cascante, Marta²  Bassilian, Sara¹  Centelles, Josep Joan²  Lee, Wai-Nang Paul¹  Rodriguez-Gil, Joan Enric³  Medrano, Antonio³ 

[1] Harbor-UCLA Research and Education Institute, UCLA School of Medicine, 1124 West Carson Street RB1, Torrance, CA 90502, USA;Department of Biochemistry and Molecular Biology, University of Barcelona, C/ Martı́ i Franqués 1, Barcelona 08028, Spain;Unit of Animal Reproduction, Department of Animal Medicine and Surgery, School of Veterinary Medicine, Autonomous University of Barcelona, Bellaterra 08193, Spain

关键词: Metabolomics;    Mass isotopomer analysis;    Glucose metabolism;    [1;    2-13C2]Glucose;    Boar spermatozoa;    Glycolysis;    GC/MS;    gas chromatography/mass spectrometry;    G6P;    glucose-6-phosphate;    PAA;    phenylacetic acid;    PC;    pyruvate carboxylase (EC 6.4.1.1);    PDH;    pyruvate dehydrogenase (EC 1.2.4.1);   

DOI  :  10.1016/S0014-5793(03)01185-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Metabolomic characteristics in boar spermatozoa were studied using [1,2-¹³C₂]glucose and mass isotopomer analysis. In boar spermatozoa, glycolysis was the main pathway of glucose utilization producing lactate/pyruvate, whereas no gluconeogenesis was seen. Slight glycogen synthesis through the direct pathway and some incorporation of pyruvate into the Krebs cycle also took place. Neither RNA ribose-5-phosphate nor fatty acid synthesis from glucose occurred despite the detection of pyruvate dehydrogenase activity. In contrast to the known metabolic activities in dog sperm, boar spermatozoa have low levels of energy production and biosynthetic activities suggesting two different metabolic profiles for the two different phenotypes.

【 授权许可】

Unknown