FEBS Letters | |
Identification of the blood group Lewisa determinant in the oviducal mucins of Xenopus tropicalis | |
Guérardel, Yann1  Kol, Ossarath1  Boujard, Daniel3  Guillet, Brigitte3  Petit, Daniel2  Madigou, Thierry3  Maes, Emmanuel1  Strecker, Gérard1  Maftah, Abderrahman2  | |
[1]Unité de Glycobiologie Structurale et Fonctionnelle, UMR 8576, USTL, 59655 Villeneuve d'Ascq Cedex, France | |
[2]Equipe de Glycobiologie et Biotechnologie, Institut des Sciences de la Vie et de la Santé, 123 Avenue Albert Thomas, 87060 Limoges, France | |
[3]UMR Interactions Cellulaires et Moléculaires, CNRS/Université de Rennes 1, Bat. 13 Campus de Beaulieu, 35042 Rennes Cedex, France | |
关键词: Lewisa antigen; O-Glycan; Mucin; Fucosyltransferase; Xenopus tropicalis; | |
DOI : 10.1016/S0014-5793(03)01183-9 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The amphibian Xenopus tropicalis appears an increasingly appealing model for both genetic and developmental biology studies, compared to the related species Xenopus laevis. Study of the glycosylation pattern of its secreted glycoproteins revealed that this species synthesizes large amounts of Lewisa epitope, whereas this motif has previously only been identified in animals within the primate lineage. The use of 1H-nuclear magnetic resonance spectroscopy enabled us to resolve the sequence of three Lewisa-bearing O-linked glycans associated with oviducal secretions, out of which one contained the novel sequence Gal(β1–3)GlcNAc(β1–6)GalNAc-ol. These structural data suggested the emergence of an α1,4-fucosyltransferase activity in animals outside the primate lineage. On this basis, the screening of a X. tropicalis GenBank database with human Lewis-fucosyltransferase sequences revealed the occurrence of a putative fucosyltransferase gene that presented an unusual acceptor motif.
【 授权许可】
Unknown
【 预 览 】
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