Raman imaging of hemozoin within the food vacuole of Plasmodium falciparum trophozoites

FEBS Letters

McNaughton, Don²  Glenister, Fiona K¹  Cooke, Brian M¹  Langford, Steven J²  Wood, Bayden R²  Lim, Janelle² 

[1] Department of Microbiology, Monash University, Clayton, Vic. 3800, Australia;Centre for Biospectroscopy and School of Chemistry, Monash University, Clayton, Vic. 3800, Australia

关键词: Malaria;    Raman spectroscopy;    Hemozoin;    β-Hematin;    Plasmodium falciparum;   

DOI  :  10.1016/S0014-5793(03)00975-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Micro-Raman spectra of hemozoin encapsulated within the food vacuole of a Plasmodium falciparum-infected erythrocyte are presented. The spectrum of hemozoin is identical to the spectrum of β-hematin at all applied excitation wavelengths. The unexpected observation of dramatic band enhancement of A_1g modes including ν₄ (1374 cm⁻¹) observed when applying 780 nm excitation enabled Raman imaging of hemozoin in the food vacuole. This unusual enhancement, resulting from excitonic coupling between linked porphyrin moieties in the extended porphyrin array, enables the investigation of hemozoin within its natural environment for the first time.

【 授权许可】

Unknown