Physical and functional interaction of the Werner syndrome protein with poly‐ADP ribosyl transferase

FEBS Letters

Kontou, Maria¹  Schweiger, Manfred¹  Adelfalk, Caroline¹  Hirsch-Kauffmann, Monica¹ 

[1] Free University of Berlin, Department of Biochemistry, Thielallee 63, D-14195 Berlin, Germany

关键词: Werner's syndrome;    WRN;    Poly-ADP ribosyl transferase;    ADP-ribosylation;    FEN-1;    flap endonuclease 1;    MRC;    multiprotein DNA replication complex;    NHEJ;    non-homologous end joining;    pADPRT;    poly-ADP ribosyl transferase;    PAGE;    polyacrylamide gel electrophoresis;    PCNA;    proliferating cell nuclear antigen;    RPA;    replication protein A;    WRN;    Werner protein;    WS;    Werner's syndrome;   

DOI  :  10.1016/S0014-5793(03)01088-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Werner's syndrome is a rare disease of premature ageing. The WRN gene product defective in this disorder belongs to the RecQ helicase family and is thought to be involved in DNA metabolism. Another protein, which plays an important role in both DNA replication and repair, is the poly-ADP ribosyl transferase. Here we demonstrate an interaction of these two proteins resulting in ADP-ribosylation of the WRN protein. These results imply that WRN is involved in DNA replication and in DNA repair.

【 授权许可】

Unknown