Subcellular localization and mechanisms of nucleocytoplasmic distribution of p202, an interferon‐inducible candidate for lupus susceptibility

FEBS Letters

Choubey, Divaker¹  Xin, Hong¹  Pramanik, Rocky¹ 

[1] Department of Radiation Oncology, Stritch School of Medicine, Loyola University Medical Center, 2160 South First Avenue, Mail Code 114B, Maywood, IL 60153, USA

关键词: Interferon;    p202;    Mitochondrial targeting;    Lupus;    Green fluorescence protein;   

DOI  :  10.1016/S0014-5793(03)01006-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Increased expression of p202 (52 kDa), an interferon (IFN)-inducible murine protein, in splenic cells (B- and T-cells) derived from female mice of the lupus-prone strains is correlated with increased susceptibility to develop systemic lupus erythematosus. However, the molecular mechanisms remain unclear. Our previous studies have indicated that, in IFN-treated fibroblasts, p202 is detected both in the cytoplasm and in the nucleus. Moreover, in the cytoplasm, a fraction of p202 associates with a membranous organelle. Here we report that, in the cytoplasm, a fraction of p202 associated with mitochondria. Additionally, we found that the constitutive p202 is primarily detected in the cytoplasm. Remarkably, the IFN treatment of cells potentiated nuclear accumulation of p202. Our observations are consistent with the possibility that IFN signaling regulates p202 levels as well as its nucleocytoplasmic distribution. These observations will serve as a basis to elucidate the molecular mechanisms by which p202 contributes to lupus susceptibility.

【 授权许可】

Unknown