Solution structure of epiregulin and the effect of its C‐terminal domain for receptor binding affinity

FEBS Letters

Sato, Katsuharu²  Kawano, Keiichi²  Miura, Kazunori²  Gomi, Tomoharu³  Aizawa, Tomoyasu²  Miyamoto, Kaoru¹  Nakamura, Takashi²  Tada, Masahito²  Mizuguchi, Mineyuki² 

[1] Department of Biochemistry, Fukui Medical University, Shimoaizuki, Matsuoka, Fukui 910-1193, Japan;Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, 2630 Sugitani, Toyama 930-0194, Japan;Scientific Instrument Center, Toyama Medical and Pharmaceutical University, 2630 Sugitani, Toyama 930-0194, Japan

关键词: Epiregulin;    Nuclear magnetic resonance;    Epidermal growth factor;    Structure;    EPR;    epiregulin;    EGF;    epidermal growth factor;    BTC;    betacellulin;    TGF;    transforming growth factor;    HB-EGF;    heparin-binding EGF-like growth factor;    HRG;    heregulin;    HRG-αe;    EGF-like domain of HRG-α;    BTCe;    EGF-like domain of BTC;    NMR;    nuclear magnetic resonance;    Trx;    thioredoxin;    DQF-COSY;    double quantum filtered correlation spectroscopy;    TOCSY;    total correlation spectroscopy;    NOESY;    nuclear Overhauser enhancement spectroscopy;    NOE;    nuclear Overhauser effect;    CSI;    chemical shift index;    SA;    simulated annealing;    RMSD;    root mean square deviation;    GBP;    growth-blocking peptide;   

DOI  :  10.1016/S0014-5793(03)01005-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Epiregulin (EPR), a novel member of epidermal growth factor (EGF) family, is a ligand for ErbB-1 and ErbB-4 receptors. The binding affinity of EPR for the receptors is lower than those of other EGF-family ligands. The solution structure of EPR was determined using two-dimensional nuclear magnetic resonance spectroscopy. The secondary structure in the C-terminal domain of EPR is different from other EGF-family ligands because of the lack of hydrogen bonds. The structural difference in the C-terminal domain may provide an explanation for the reduced binding affinity of EPR to the ErbB receptors.

【 授权许可】

Unknown