The oxidant‐responsive diaphorase of Rhodobacter capsulatus is a ferredoxin (flavodoxin)‐NADP(H) reductase

FEBS Letters

Cortez, Néstor¹  Armesto, Martín¹  Carrillo, Néstor¹  Bittel, Cristian¹  Tabares, Leandro C.¹ 

[1] Instituto de Biologı́a Molecular y Celular de Rosario, Universidad Nacional de Rosario and CONICET, Suipacha 531, S2002LRK Rosario, Argentina

关键词: Ferredoxin (flavodoxin)-(reduced) nicotinamide adenine dinucleotide phosphate reductase;    Ferredoxin;    Flavodoxin;    Oxidative stress;    Rhodobacter capsulatus;   

DOI  :  10.1016/S0014-5793(03)01075-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Challenge of Rhodobacter capsulatus cells with the superoxide propagator methyl viologen resulted in the induction of a diaphorase activity identified as a member of the ferredoxin (flavodoxin)-(reduced) nicotinamide adenine dinucleotide phosphate (NADP(H)) reductase (FPR) family by N-terminal sequencing. The gene coding for Rhodobacter FPR was cloned and expressed in Escherichia coli. Both native and recombinant forms of the enzyme were purified to homogeneity rendering monomeric products of ∼30 kDa with essentially the same spectroscopic and kinetic properties. They were able to bind and reduce Rhodobacter flavodoxin (NifF) and to mediate typical FPR activities such as the NADPH-driven diaphorase and cytochrome c reductase.

【 授权许可】

Unknown