Inhibition of influenza A virus sialidase activity by sulfatide

FEBS Letters

Fujii, Satoshi¹  Miyamoto, Daisei²  Murakami, Masanori¹  Suzuki, Takashi²  Suzuki, Yasuo²  Hidari, Kazuya I.-P.Jwa²  Li, Yu-Teh³  Nishinaka, Daisuke²  Takahashi, Tadanobu² 

[1] Department of Physical Chemistry, University of Shizuoka, School of Pharmaceutical Sciences, Shizuoka 422-8526, Japan;Department of Biochemistry, University of Shizuoka, School of Pharmaceutical Sciences, CREST, JST, and COE Program in the 21st Century, Shizuoka 422-8526, Japan;Department of Biochemistry, Tulane University School of Medicine, New Orleans, LA 70112, USA

关键词: Sulfatide;    Sialidase;    Influenza virus;    Neuraminidase;    Inhibitor;    4G-Neu5Ac2en;    4-guanidino-2-deoxy-2;    3-didehydro-N-acetyl-D-neuraminic acid;    HA;    hemagglutinin;    MDCK;    Madin–Darby canine kidney;    4-MU-Neu5Ac;    2′-(4-methylumbelliferyl)-α-D-N-acetylneuraminic acid;    4-MU;    4-methylumbelliferone;    NA;    neuramindase;    Neu5Ac2en;    2-deoxy-2;    3-didehydro-N-acetyl-D-neuraminic acid;   

DOI  :  10.1016/S0014-5793(03)01045-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Sulfatide, which binds to influenza A viruses and prevents the viral infection, was found to inhibit the sialidase activities of influenza A viruses in a pH-dependent manner. The kinetic parameters of the effect of sulfatide on the sialidase activities of human influenza A viruses using fluorometric assay indicated that sulfatide was a powerful and non-competitive type inhibitor in low-pH conditions.

【 授权许可】

Unknown