Structural requirements for nuclear localization of GCMa/Gcm‐1

FEBS Letters

Kilian, Karin¹  Kardash, Elena¹  Wegner, Michael¹  Stamminger, Thomas²  Hashemolhosseini, Said¹  Lischka, Peter² 

[1] Institut für Biochemie, Universität Erlangen-Nürnberg, Fahrstrasse 17, D-91054 Erlangen, Germany;Institut für Klinische und Molekulare Virologie, Universität Erlangen-Nürnberg, D-91054 Erlangen, Germany

关键词: Nuclear localization sequence;    Nuclear export signal;    Nuclear import;    Nucleocytoplasmic shuttling;    Glial cells missing;   

DOI  :  10.1016/S0014-5793(03)01037-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

GCM proteins constitute a small transcription factor family. Nuclear localization of Drosophila GCM is mediated by a typical bipartite nuclear localization sequence (NLS) close to the DNA-binding GCM domain. Here, we have analyzed nuclear localization of the mammalian GCM proteins. Whereas GCMb/Gcm-2 contained a classical bipartite NLS, nuclear localization of GCMa/Gcm-1 was mediated by two regions without resemblance to known NLS, one corresponding to the amino-terminal part of the GCM domain, the second defined as a tyrosine-and-proline-rich carboxy-terminal region. Nuclear import was counteracted by an amino-terminal nuclear export activity. This complex regulation of subcellular localization has important implications for GCMa/Gcm-1 function.

【 授权许可】

Unknown