Interferon alpha inhibits the nuclear factor kappa B activation triggered by X gene product of hepatitis B virus in human hepatoma cells

FEBS Letters

Eguchi, Katsumi¹  Ohata, Kazuyuki¹  Nakao, Kazuhiko²  Ishikawa, Hiroki¹  Ichikawa, Tatsuki¹  Shigeno, Masaya¹  Hamasaki, Keisuke¹  Nishimura, Daisuke¹ 

[1] The First Department of Internal Medicine, Nagasaki University, 1-7-1 Sakamoto, Nagasaki 852-8501, Japan;Health Research Center, Nagasaki University, 1-7-1 Sakamoto, Nagasaki 852-8501, Japan

关键词: Hepatitis B virus X protein;    Nuclear factor kappa B;    Interferon alpha;    IFN-α;    interferon alpha;    IFN-γ;    interferon gamma;    NF-κB;    nuclear factor kappa B;    IKK;    IκB kinase;    NIK;    NF-κB-inducing kinase;    HBV;    hepatitis B virus;    HBx;    X gene product of HBV;    HCC;    hepatocellular carcinoma;   

DOI  :  10.1016/S0014-5793(03)01034-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

X gene product of hepatitis B virus (HBV) (HBx) regulates many transcription factors including nuclear factor kappa B (NF-κB) and plays a key role in hepatocarcinogenesis. In this study, we demonstrated that the expression of full HBV genome and HBx gene similarly stimulated the transcriptional activity of NF-κB in HuH-7 human hepatoma cells, and that interferon (IFN)-α as well as dominant negative mutant of IκB kinase-α effectively inhibited the HBx-mediated NF-κB activation, but IFN-γ did not. These results suggest that IFN-α may have a function to block the NF-κB activating pathway triggered by HBx in HBV hepatocytes.

【 授权许可】

Unknown