α‐Melanocyte‐stimulating hormone inhibits lipopolysaccharide‐induced biological responses by downregulating CD14 from macrophages

FEBS Letters

Sarkar, Abira¹  Manna, Sunil K¹  Sreenivasan, Yashin¹ 

[1] Laboratory of Immunology, Centre for DNA Fingerprinting and Diagnostics, Nacharam, Hyderabad 500 076, India

关键词: CD14;    α-Melanocyte stimulating hormone;    Lipopolysaccharide;    Nuclear transcription factor κB;    Macrophage;    KSCN;    potassium thiocyanate;    ICAM;    intracellular adhesion molecule;    MC-1R;    melanocortin 1 receptor;    α-MSH;    α-melanocyte-stimulating hormone;    NBT;    nitroblue tetrazolium;    NF-κB;    nuclear transcription factor κB;    SA-LPS;    serum-activated lipopolysaccharide;    SEAP;    secretory alkaline phosphatase;   

DOI  :  10.1016/S0014-5793(03)01029-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Monocytes/macrophages are the first cells involved in inflammation. α-Melanocyte-stimulating hormone (α-MSH) is known to possess an anti-inflammatory role induced by a variety of stimuli; however, the molecular mechanisms underlying these effects are not clearly defined. In this report we provide evidence that α-MSH inhibited serum-activated lipopolysaccharide (SA-LPS)-induced proteolytic enzyme release, oxidative burst response, reactive oxygen intermediate generation, nitric oxide production, and adhesion molecule expression in monocyte-derived macrophages. α-MSH also inhibited SA-LPS-induced nuclear transcription factor κB activation not only in macrophages, but also in a T-cell line and human neutrophils isolated from fresh blood. α-MSH downregulated CD14, but not interleukin-1 receptor, tumor necrosis factor receptor 1 or 2 from the surface of macrophages. Anti-CD14 antibody was unable to protect α-MSH-mediated downregulation of CD14. Overall, our results suggest that α-MSH exerts its anti-inflammatory effect by a novel mechanism in macrophages through downregulating of the endotoxin receptor CD14.

【 授权许可】

Unknown