Anti‐herbicide single‐chain antibody expression confers herbicide tolerance in transgenic plants

FEBS Letters

Yamaguchi, Isomaro¹  Suzuki, Yoshihito¹  Ohkawa, Hideo²  Eto, Junichi¹ 

[1] Department of Applied Biological Chemistry, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan;Division of Bioscience, Graduate School of Science and Technology, Kobe University, Rokkodai-cho 1-1 Nada-ku, Kobe 657-8501, Japan

关键词: Chlorpropham;    Single-chain antibody;    Herbicide tolerance;    Glycosylphosphatidylinositol anchor;    Arabidopsis thaliana;   

DOI  :  10.1016/S0014-5793(03)00871-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

An anti-chlorpropham single-chain variable-fragment (scFv) gene was introduced into Arabidopsis in a manner to express the antibody fragment in each of four different subcellular compartments. The accumulation of scFv in transgenic plants was detected by targeting the fragment in the endoplasmic reticulum or apoplastic space, or by expressing the fragment as a glycosylphosphatidylinositol-anchored protein, while no accumulation could be detected by targeting the fragment in the cytosol. Transgenic plants accumulating the scFv gene at a high level in the endoplasmic reticulum had enhanced tolerance to chlorpropham in comparison with the non-transformants.

【 授权许可】

Unknown