New members of the tomato ERF family show specific expression pattern and diverse DNA‐binding capacity to the GCC box element

FEBS Letters

Regad, Farid²  Sanchez-Ballesta, Maria Theresa²  Pech, Jean-Claude²  Latché, Alain²  Jones, Brian²  Tournier, Barthélémy²  Pesquet, Edouard¹  Bouzayen, Mondher² 

[1] UMR 5546 CNRS/UPS, Pole de Biotechnologie Végétale, 24 Chemin de Borde Rouge, Auzeville P.O. Box 17, 31326 Castanet Tolosan Cedex, France;UMR 990 INRA/INP-ENSAT, Pole de Biotechnologie Végétale, 24 Chemin de Borde Rouge, P.O. Box 107, 31326 Castanet Tolosan Cedex, France

关键词: Ethylene response factor;    3D modeling;    Lycopersicon esculentum;   

DOI  :  10.1016/S0014-5793(03)00757-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Four new members of the ERF (ethylene-response factor) family of plant-specific DNA-binding (GCC box) factors were isolated from tomato fruit (LeERF1–4). Phylogenetic analysis indicated that LeERF2 belongs to a new ERF class, characterized by a conserved N-terminal signature sequence. Expression patterns and cis/trans binding affinities differed between the LeERFs. Combining experimental data and modeled three-dimensional analysis, it was shown that binding affinity of the LeERFs was affected by both the variation of nucleotides surrounding the DNA cis-element sequence and the nature of critical amino acid residues within the ERF domain.

【 授权许可】

Unknown