Silymarin inhibits TNF‐α‐induced expression of adhesion molecules in human umbilical vein endothelial cells

FEBS Letters

Kang, Jong Soon¹  Park, Song-Kyu¹  Kim, Hwan Mook¹  Yang, Kyu-Hwan¹ 

[1] Biopotency Evaluation Laboratory, Korea Research Institute of Bioscience and Biotechnology, Taejon 305-333, South Korea

关键词: Silymarin;    VCAM-1;    ICAM-1;    E-selectin;    NF-κB/Rel;    VCAM-1;    vascular cell adhesion molecule-1;    ICAM-1;    intercellular adhesion molecule-1;    HUVECs;    human umbilical vein endothelial cells;    NF-κB/Rel;    nuclear factor-κB/Rel;    EMSA;    electrophoretic mobility shift assay;    RT-PCR;    reverse transcription-polymerase chain reaction;   

DOI  :  10.1016/S0014-5793(03)00827-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Silymarin is known to have an anti-atherosclerotic activity, but the mechanism responsible for it remains unclear. Here, we demonstrate a possible mechanism involved in the anti-atherosclerotic activity of silymarin. Silymarin inhibited THP-1 cell adhesion to human umbilical vein endothelial cells (HUVECs). Silymarin also suppressed the TNF-α-induced protein and mRNA expression of adhesion molecules, such as VCAM-1, ICAM-1 and E-selectin, in HUVECs. Moreover, silymarin suppressed the TNF-α-induced DNA binding of NF-κB/Rel in HUVECs. Taken together, these results demonstrate that silymarin exerts an anti-atherosclerotic activity, at least in part, by inhibiting the expression of adhesion molecules.

【 授权许可】

Unknown