Molecular and functional characterisation of the zebrafish (Danio rerio) PEPT1‐type peptide transporter

FEBS Letters

Maffia, Michele¹  Storelli, Carlo¹  Peric, Mark²  Tiso, Natascia³  Kottra, Gabor²  Daniel, Hannelore²  Boll, Michael²  Argenton, Francesco³  Romano, Alessandro¹  Verri, Tiziano¹ 

[1] Laboratory of General Physiology, Department of Biological and Environmental Sciences and Technologies, University of Lecce, via Provinciale Lecce-Monteroni, I-73100 Lecce, Italy;Institute of Nutritional Sciences, Technical University of Munich, Hochfeldweg 2, D-85350 Freising-Weihenstephan, Germany;Department of Biology, University of Padua, via U. Bassi 58/B, I-35131 Padua, Italy

关键词: Intestine;    Peptide transport;    Two-electrode voltage clamp;    Whole-mount in situ hybridisation;    Gut morphogenesis;    Xenopus laevis oocytes;    dpf;    days post-fertilisation;    PEPT1;    peptide transporter 1;    PEPT2;    peptide transporter 2;    PTR family;    peptide transporter family;    TEVC;    two-electrode voltage clamp;   

DOI  :  10.1016/S0014-5793(03)00759-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We report the molecular and functional characterisation of a novel peptide transporter from zebrafish, orthologue to mammalian and avian PEPT1. Zebrafish PEPT1 is a low-affinity/high-capacity system. However, in contrast to higher vertebrate counterparts in which maximal transport activity is independent of extracellular pH, zebrafish PEPT1 maximal transport rates unexpectedly increase at alkaline extracellular pH. Zebrafish pept1 is highly expressed in the proximal intestine since day 4 post-fertilisation, thus preceding functional maturation of the gut, first feeding and complete yolk resorption. Zebrafish PEPT1 might help to understand the evolutionary and functional relationships among vertebrate peptide transporters. Moreover, zebrafish pept1 can be a useful marker for screening mutations that affect gut regionalisation, differentiation and morphogenesis.

【 授权许可】

Unknown