Unidirectional effect of lauryl sulfate on the reversible NADH:ubiquinone oxidoreductase (Complex I)

FEBS Letters

Grivennikova, V.G¹  Cecchini, G²  Ushakova, A.V¹  Vinogradov, A.D¹ 

[1] Department of Biochemistry, School of Biology, Moscow State University, Moscow 119992, Russia;Molecular Biology Division, Veterans Affairs Medical Center, San Francisco, CA 94121, USA

关键词: NADH:quinone oxidoreductase;    Complex I;    Lauryl sulfate;    Unidirectional inhibition;    Electrochemical coupling;    SMP;    submitochondrial particles;    LS;    lauryl sulfate;    RCR;    respiratory control ratio;   

DOI  :  10.1016/S0014-5793(03)00765-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Lauryl sulfate inhibits the Δ _H ⁺-dependent reverse electron transfer reactions catalyzed by NADH:ubiquinone oxidoreductase (Complex I) in coupled bovine heart submitochondrial particles and in vesicles derived from Paracoccus denitrificans. The inhibitor affects neither NADH oxidase (coupled or uncoupled) nor NADH:ferricyanide reductase and succinate oxidase activities at the concentrations that selectively prevent the succinate-supported, rotenone-sensitive NAD⁺ or ferricyanide reduction. Possible uncoupling effects of the inhibitor are ruled out: in contrast to oligomycin and gramicidin, which increases and decreases the rate of the reverse electron transfer, respectively, in parallel with their coupling and uncoupling effects, lauryl sulfate does not affect the respiratory control ratio. A mechanistic model for the unidirectional effect of lauryl sulfate on the Complex I catalyzed oxidoreduction is proposed.

【 授权许可】

Unknown