Unraveling thioredoxin‐linked metabolic processes of cereal starchy endosperm using proteomics

FEBS Letters

Cai, Nick²  Tanaka, Charlene K¹  Vensel, William H¹  Hurkman, William J¹  Balmer, Yves²  Wong, Joshua H²  Buchanan, Bob B² 

[1] USDA, Agricultural Research Service, Western Regional Research Center, Albany, CA 94710, USA;Department of Plant and Microbial Biology, 111 Koshland Hall, University of California, Berkeley, CA 94720, USA

关键词: Thioredoxin;    Redox regulation;    Wheat;    Triticum aestivum;    Endosperm;    Proteomics;    GSH;    reduced glutathione;    DTT;    dithiothreitol;    mBBr;    monobromobimane;    PFP;    fructose 6-phosphate 1-phosphotransferase;    PPi;    inorganic pyrophosphate;    PDI;    protein disulfide isomerase;    PPDK;    pyruvate;    Pi dikinase;   

DOI  :  10.1016/S0014-5793(03)00696-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Application of a thiol-specific probe, monobromobimane, with proteomics and enzyme assays led to the identification of 23 thioredoxin targets in the starchy endosperm of mature wheat seeds (Triticum aestivum cv. Butte), almost all containing at least two conserved cysteines. The identified targets, 12 not known to be thioredoxin-linked, function in a spectrum of processes: metabolism (12 targets), protein storage (three), oxidative stress (three), protein degradation (two), protein assembly/folding (one) and unknown reactions (two). In addition to formulating metabolic pathways functional in the endosperm, the results suggest that thioredoxin acts in redox regulation throughout the life cycle of the seed.

【 授权许可】

Unknown