Interaction of EBV latent origin of replication with the nuclear matrix: identification of S/MAR sequences and protein components

FEBS Letters

Mattia, Elena¹  Chichiarelli, Silvia²  D'Erme, Maria²  Masciarelli, Silvia¹  Ferraro, Anna²  Zampieri, Michele¹  Mearini, Giulia¹ 

[1] Department of Public Health Sciences, University ‘La Sapienza’, Rome, Italy;Department of Biochemical Sciences, University ‘La Sapienza’, Rome, Italy

关键词: Epstein Barr virus;    Nuclear matrix;    S/MAR;    Cross-linking;   

DOI  :  10.1016/S0014-5793(03)00690-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

During latency, Epstein Barr virus (EBV) genome, as an episome, is attached to the nuclear matrix (NM) via the latent origin of replication ori P. Within this element, we have found that a region, 580 bp long, encompassing the replicator DS element, shows the strongest affinity for the NM. In addition, by cross-linking with cis-diamminedichloroplatinum, we have identified two NM proteins with an apparent molecular weight of 85 and 60 kDa that, with high affinity and specificity, bind ori P. These proteins are not induced by EBV infection, but their interaction with ori P is lost upon induction of EBV lytic cycle. These data strongly suggest that the binding of ori P to specific components of the NM is required for EBV latent replication.

【 授权许可】

Unknown