Modulation of Cav3.2 T‐type Ca2+ channels by protein kinase C

FEBS Letters

Perez-Reyes, Edward¹  Jeong, Seong-Woo³  Lee, Jung-Ha²  Park, Jin-Yong² 

[1] Department of Pharmacology, University of Virginia, Charlottesville, VA 22908, USA;Department of Life Science, Sogang University, Shinsu-dong, Mapo-Gu, Seoul 121-742, South Korea;Department of Physiology, Yonsei University Wonju College of Medicine, Ilsan-Dong 162, Wonju, Kangwon-Do, South Korea

关键词: T-type calcium channel;    α1H;    Protein kinase C;    Phorbol-12-myristate-13-acetate;    Modulation;   

DOI  :  10.1016/S0014-5793(03)00665-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Although T-type Ca²⁺ channels have been implicated in numerous physiological functions, their regulations by protein kinases have been obscured by conflicting reports. We investigated the effects of protein kinase C (PKC) on Ca_v3.2 T-type channels reconstituted in Xenopus oocytes. Phorbol-12-myristate-13-acetate (PMA) strongly enhanced the amplitude of Ca_v3.2 channel currents (∼3-fold). The augmentation effects were not mimicked by 4α-PMA, an inactive stereoisomer of PMA, and abolished by preincubation with PKC inhibitors. Our findings suggest that PMA upregulates Ca_v3.2 channel activity via activation of oocyte PKC.

【 授权许可】

Unknown