Stepping motion of the organelle in a perfused characean cell

FEBS Letters

Kagawa, Yuki¹  Tsuchiya, Yoshimi¹ 

[1] Department of Biological Sciences, Tokyo Institute of Technology, Yokohama 226-8501, Japan

关键词: Stepping motion;    Organelle;    Actin;    Step size;    Nitella;    Chara myosin;    APW;    artificial pond water;    PDF;    pairwise distance distribution function;   

DOI  :  10.1016/S0014-5793(03)00574-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The sliding motion of organelles in a perfused characean internodal cell has been investigated. A small number of myosin molecules attached to the organelle are known to slide along actin bundles lying on the inner surface of the cell. The stepping motion of the organelles was first observed using a high-speed camera with a time resolution of 1 ms. The spatial frequency of all pairwise differences in displacement of the stepping motion has been estimated to be 0.012–0.014 nm⁻¹, which implies that the organelle moves with large steps of 71–83 nm along the actin bundles in a perfused characean cell.

【 授权许可】

Unknown