Fibroblast growth factor‐10 upregulates Na,K‐ATPase via the MAPK pathway

FEBS Letters

Kamp, D.W¹  Sznajder, J.I¹  Comellas, A¹  Lecuona, E¹  Upadhyay, D¹ 

[1] Division of Pulmonary and Critical Care Medicine, Northwestern University Medical School, Chicago, IL 60611, USA

关键词: Fibroblast growth factor-10;    Mitogen-activated protein kinase;    Na;    K-ATPase;    Alveolar epithelial cell;    AEC;    alveolar epithelial cells;    FGF-10;    fibroblast growth factor-10;    MAPK;    mitogen-activated protein kinase;   

DOI  :  10.1016/S0014-5793(03)00527-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We studied the effects of fibroblast growth factor (FGF-10) on alveolar epithelial cell (AEC) Na,K-ATPase regulation. Within 30 min FGF-10 increased Na,K-ATPase activity and α1 protein abundance by 2.5-fold at the AEC plasma membrane. Pretreatment of AEC with the mitogen-activated protein kinase (MAPK) inhibitor U0126, a Grb2-SOS inhibitor (SH3-b-p peptide), or a Ras inhibitor (farnesyl transferase inhibitor (FTI 277)), as well as N17-AEC that express a Ras dominant negative protein each prevented FGF-10-mediated Na,K-ATPase recruitment to the AEC plasma membrane. Accordingly, we provide first evidence that FGF-10 upregulates (short-term) the Na,K-ATPase activity in AEC via the Grb2-SOS/Ras/MAPK pathway.

【 授权许可】

Unknown