Defined subcomplexes of the A1 ATPase from the archaeon Methanosarcina mazei Gö1: biochemical properties and redox regulation

FEBS Letters

Lemker, Thorsten²  Grüber, Gerhard¹  Müller, Volker²  Schmid, Roland³ 

[1] FR 2.5-Biophysik, Universität des Saarlandes, D-66421 Homburg, Germany;Section Microbiology, Department Biology I, Ludwig-Maximilians-University, Maria-Ward-Str. 1a, D-80638 Munich, Germany;FB5, AG Mikrobiologie, Universität Osnabrück, Barbarastrasse 11, 49069 Osnabrück, Germany

关键词: Archaeon;    A1 ATPase;    Subcomplex;    Kinetic property;    Regulation;    Methanosarcina mazei Gö1;   

DOI  :  10.1016/S0014-5793(03)00496-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The potential A₁ ATPase genes ahaA, ahaB, ahaC, ahaD, ahaE, ahaF, and ahaG from the anaerobic archaeon Methanosarcina mazei Gö1 were overexpressed in Escherichia coli DK8 (pTL2). An A₁ complex was purified to apparent homogeneity and shown by Western blot and N-terminal sequence analyses to contain subunits A, B, C, D, and F but to be devoid of subunits E and G. Further removal of subunit C was without effect on ATPase activity. The enzyme was most active at pH 5.2 and required bisulfite and acetate for maximal activity. Kinetic studies confirmed three new inhibitors for A₁ ATPases (diethylstilbestrol and its derivatives hexestrol and dienestrol) and identified redox modulation as a new type of regulation of archaeal A₁ ATPases.

【 授权许可】

Unknown