Transient increase of phosphatidylcholine in plant cells in response to phosphate deprivation

FEBS Letters

Block, Maryse A¹  Maréchal, Eric¹  Joyard, Jacques¹  Bligny, Richard¹  Jouhet, Juliette¹ 

[1] Laboratoire de Physiologie Cellulaire Végétale, UMR 5019 (CNRS/CEA/Université Joseph Fourier), DRDC/PCV, CEA-Grenoble, 17 rue des Martyrs, F-38054 Grenoble cedex 9, France

关键词: Plant cell;    Phosphate deprivation;    Membrane;    Galactolipid;    Phosphatidylcholine;    Chloroplast;    FA;    fatty acid;    DGDG;    digalactosyldiacylglycerol;    MGDG;    monogalactosyldiacylglycerol;    PC;    phosphatidylcholine;    DAG;    diacylglycerol;    PE;    phosphatidylethanolamine;    DPG;    cardiolipin;    PG;    phosphatidylglycerol;    PI;    phosphatidylinositol;    FAME;    fatty acid methylated ester;    Pi;    inorganic phosphate;    Cells grown without Pi are qualified as ‘−Pi’ whereas those grown with 4 mM (for Acer pseudoplatanus) and 1 mM (for Arabidopsis thaliana) of Pi are considered as ‘+Pi’ controls;   

DOI  :  10.1016/S0014-5793(03)00477-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In plants, phosphate deprivation is normally known to decrease the phospholipid content consistent with a mobilization of the phosphate reserve, and conversely to increase non-phosphorous membrane lipids such as digalactosyldiacylglycerol. We report here that unexpectedly, at an early stage of phosphate starvation, phosphatidylcholine (PC) increases transiently. We also show that a significant pool of diacylglycerol (DAG) with the same fatty acid composition as that of PC is present and moreover increases in response to phosphate deprivation. The evolution of the molecular profile of the newly synthesized galactolipids is compatible with a utilization of DAG accumulating from PC hydrolysis, achieved after selection of their acyl molecular species by the galactolipid synthesizing enzymes.

【 授权许可】

Unknown