Characterization of thioredoxin y, a new type of thioredoxin identified in the genome of Chlamydomonas reinhardtii

FEBS Letters

Lemaire, S.D.²  Collin, V.²  Miginiac-Maslow, M.²  Keryer, E.²  Quesada, A.¹ 

[1] Departamento de Bioquı́mica y Biologı́a Molecular, Faculdad de Veterinaria, Avenida de la Universidad s/n, 10071 Cáceres, Spain;Institut de Biotechnologie des Plantes, UMR 8618 CNRS, Université Paris-Sud, Bâtiment 630, 91405 Orsay Cedex, France

关键词: Fructose-1;    6-bisphosphate phosphatase;    NADP-malate dehydrogenase;    Peroxiredoxin;    Thioredoxin;    Chlamydomonas reinhardtii;    EST;    expressed sequence tag;    FBPase;    fructose-1;    6-bisphosphate phosphatase;    NADP-MDH;    NADP-malate dehydrogenase;    2-cys PRX;    2-cys peroxiredoxin;    TRX;    thioredoxin;    NTR;    NADPH thioredoxin reductase;   

DOI  :  10.1016/S0014-5793(03)00416-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The sequencing of the Arabidopsis genome revealed a multiplicity of thioredoxins (TRX), ubiquitous protein disulfide oxido-reductases. We have analyzed the TRX family in the genome of the unicellular green alga Chlamydomonas reinhardtii and identified eight different thioredoxins for which we have cloned and sequenced the corresponding cDNAs. One of these TRXs represents a new type that we named TRX y. This most probably chloroplastic TRX is highly conserved in photosynthetic organisms. The biochemical characterization of the recombinant protein shows that it exhibits a thermal stability profile and specificity toward target enzymes completely different from those of TRXs characterized so far.

【 授权许可】

Unknown