Rho kinase is required for CCR7‐mediated polarization and chemotaxis of T lymphocytes

FEBS Letters

Bardi, Giuseppe¹  Loetscher, Pius¹  Niggli, Verena² 

[1] Theodor Kocher Institute, P.O. Box, CH-3000 Bern 9, Switzerland;Department of Pathology, University of Bern, Murtenstr. 31, P.O. Box 62, CH-3010 Bern, Switzerland

关键词: Rho kinase;    Extracellular signal-regulated kinase-2;    Polarization;    Chemotaxis;    Chemokine;    Chemokine receptor;    CCL;    CC chemokine ligand;    CCR;    CC chemokine receptor;    ELC;    EBI1 ligand chemokine;    SLC;    secondary lymphoid-tissue chemokine;    ERK;    extracellular signal-regulated kinase;    ROCK;    Rho-associated coiled-coil forming protein kinase;   

DOI  :  10.1016/S0014-5793(03)00351-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We studied the role of Rho kinase and extracellular signal-regulated kinase (ERK)-2 in the polarization and migration of T lymphocytes in response to the CCR7 ligands EBI1 ligand chemokine (ELC; CCL19) and secondary lymphoid-tissue chemokine (SLC; CCL21). Both Rho kinase protein isoforms are expressed in T lymphocytes. Inhibition of the Rho kinases with Y-27632 strongly inhibited SLC- and ELC-induced polarized morphology and chemotaxis of T lymphocytes. Although the chemokines induced ERK-2 activation, the blockade of this signaling pathway showed no effect on polarization and migration. This study indicates an important role of Rho kinase in CCR7-mediated polarization and migration of T lymphocytes, whereas ERK-2 is not involved in these processes.

【 授权许可】

Unknown