DNA target sequence and FNR‐dependent gene expression

FEBS Letters

Green, Jeffrey¹  Partridge, Jonathan D¹  Stephenson, James R¹  Scott, Colin¹ 

[1] Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, Sheffield S10 2TN, UK

关键词: cAMP receptor protein-FNR family;    Oxygen sensing;    Transcriptional regulation;   

DOI  :  10.1016/S0014-5793(03)00312-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

FNR proteins are global transcription regulators that respond to fluctuations in environmental oxygen. They recognise a DNA target consisting of an inverted repeat, TTGATN₁N₂N₃N₄ATCAA (where N_1–4 represents a non-conserved tetrad, NCT). Analysis of 68 known and predicted FNR sites from the Escherichia coli K12 genome revealed a bias toward A or T at positions N₂ and N₃ of the NCT. The effect of the NCT sequence on FNR-dependent transcription in vivo was assessed using a series of class II and class I model promoters with different NCT sequences. Changing the NCT sequence did not affect basal activity but altered anaerobic induction by as much as an order of magnitude. Thus, the NCT sequence is a fundamental component in setting the dynamic range of the FNR switch.

【 授权许可】

Unknown