Cytotoxicity of ribosome‐inactivating protein saporin is not mediated through α2‐macroglobulin receptor

FEBS Letters

Hosur, M.V¹  Bagga, Shveta²  Batra, Janendra K² 

[1] Solid State Physics Division, Bhabha Atomic Research Centre, Trombay, Mumbai, India;Immunochemistry Laboratory, National Institute of Immunology, Aruna Asaf Ali Road, New Delhi 110067, India

关键词: Ribosome-inactivating protein;    Toxin;    Ribosome;    Immunotoxin;    Ribotoxin;   

DOI  :  10.1016/S0014-5793(03)00280-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Saporin is a single chain ribosome-inactivating protein produced by the plant Saponaria officinalis. Several isoforms of saporin have been isolated from various parts of the plant. In the present study recombinant saporin isoforms 5 and 6 were produced in Escherichia coli. Saporin-6 was found to be more active than saporin-5 in its N-glycosidase, cytotoxic, and genomic DNA fragmentation activities. Earlier, saporin has been shown to bind low-density lipoprotein receptor-related protein (LRP), however, in this study the sensitivities of LRP-negative and LRP-positive cell lines were found to be similar towards saporin-6 toxicity suggesting the internalization of saporin not to be solely dependent on the expression of LRP on eukaryotic cells.

【 授权许可】

Unknown