An initiator and its flanking elements function as a core promoter driving transcription of the Hepatopoietin gene

FEBS Letters

Li, Li¹  Tang, Fei¹  Zhang, Lingqiang¹  Xing, Guichun¹  Zhu, Yunping¹  He, Fuchu¹  Cheng, Jingwei¹  Zhao, Yanlin¹  Wei, Handong¹ 

[1] Department of Proteomics and Genomics, Beijing Institute of Radiation Medicine, Chinese Human Genome Center at Beijing, 27 Taiping Road, Beijing 100850, PR China

关键词: Core promoter;    Constitutive expression;    Initiator-dependent flanking element;    HPO;    hepatopoietin;    EMSA;    electrophoretic mobility shift assay;    Inr;    initiator;    DPE;    downstream promoter element;    IFE;    initiator-dependent flanking element;    BRE;    TFIIB recognition element;   

DOI  :  10.1016/S0014-5793(03)00158-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Hepatopoietin (HPO)/ALR (augmenter of liver regeneration), as a versatile hepatotrophic growth factor and a cellular thiol oxidase, is involved in a wide variety of basic processes of various tissues, especially in liver and testis. Here, we studied the regulation of HPO gene expression. By sequential deletion of the HPO 5′-flanking region, the minimal promoter of the HPO gene was shown to span positions −22 to +42 relative to the transcriptional start point. Further transfection assay and mutation analysis showed that the core promoter contains a functional initiator. Interestingly, three tandem repeats of a CTGGAGGC element, surrounding the transcription start site and bound by specific nuclear factors, were found to be pivotal for the promoter activity. This initiator flanking element functions in an initiator-dependent fashion and is present in many initiator-containing genes. Taken together, our findings revealed that the initiator-like element and its flanking repeat sequence comprise a core promoter and drive the transcriptional initiation of the HPO gene in a combinatorial manner. The HPO gene promoter might represent a novel architecture for core promoters.

【 授权许可】

Unknown