The acarbose‐biosynthetic enzyme AcbO from Actinoplanes sp. SE 50/110 is a 2‐epi‐5‐epi‐valiolone‐7‐phosphate 2‐epimerase

FEBS Letters

Altenbach, Hans-Josef²  Wehmeier, Udo F¹  Piepersberg, Wolfgang¹  Podeschwa, Michael²  Zhang, Chang-Sheng¹ 

[1] Institute of Chemical Microbiology, Bergische University, Gauss-Str. 20, D-42097 Wuppertal, Germany;Institute of Organic Chemistry, Bergische University, Gauss-Str. 20, D-42097 Wuppertal, Germany

关键词: Actinoplanes sp.;    Acarbose;    Biosynthesis;    C7-cyclitol;   

DOI  :  10.1016/S0014-5793(03)00221-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The C7-cyclitol 2-epi-5-epi-valiolone is the first precursor of the cyclitol moiety of the α-glucosidase inhibitor acarbose in Actinoplanes sp. SE50. The 2-epi-5-epi-valiolone becomes phosphorylated at C7 by the ATP dependent kinase AcbM prior to the next modifications. Preliminary data gave evidences that the AcbO protein could catalyse the first modification step of 2-epi-5-epi-valiolone-7-phosphate. Therefore, the AcbO protein, the encoding gene of which is also part of the acbKMLNOC operon, was overproduced and purified. Indeed the purified protein catalysed the 2-epimerisation of 2-epi-5-epi-valiolone-7-phosphate. The chemical structure of the purified reaction product was proven by nuclear magnetic resonance spectroscopy to be 5-epi-valiolone-7-phosphate.

【 授权许可】

Unknown