Constitutive overexpression of barley 4‐hydroxyphenylpyruvate dioxygenase in tobacco results in elevation of the vitamin E content in seeds but not in leaves

FEBS Letters

Falk, Jon¹  Andersen, Gaby¹  Krupinska, Karin¹  Kernebeck, Birgit² 

[1] Institute of Botany, University of Kiel, Olshausenstrasse 40, D-24098 Kiel, Germany;Institute of Botany, University of Cologne, Gyrhofstrasse 15, D-50931 Cologne, Germany

关键词: 4-Hydroxyphenylpyruvate dioxygenase;    Plastoquinone;    Vitamin E;    Tocopherol;    Tobacco;    Senescence;    HPPD;    4-hydroxyphenylpyruvate dioxygenase;    HGA;    homogentisate;    γ-TMT;    γ-tocopherol-methyltransferase;   

DOI  :  10.1016/S0014-5793(03)00166-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

With the aim to enhance the plant vitamin E content, the barley gene encoding 4-hydroxyphenylpyruvate dioxygenase was overexpressed in tobacco plants under control of the 35S promoter. Transgenic lines have a higher capacity for homogentisate biosynthesis as evident by a more than 10-fold higher resistance towards the bleaching herbicide sulcotrione. Seeds from transgenic lines have an up to two-fold enhanced level of vitamin E without a change in the ratio of γ-tocopherol and γ-tocotrienol. While the vitamin E content is not affected in leaves, the level of plastoquinone is enhanced in leaves of transgenic lines during leaf senescence.

【 授权许可】

Unknown