Human T‐cell leukemia virus type 1 Tax protein stimulates the interferon‐responsive enhancer element via NF‐κB activity

FEBS Letters

Fujisawa, Jun-ichi¹  Fukuhara, Shirou²  Shimizu, Toshiki¹  Li, Qin-Hua¹  Kawakita, Shigenari¹ 

[1] Department of Microbiology, Kansai Medical University, Fumizono-cho 10-15, Moriguchi, Osaka, Japan;First Department of Internal Medicine, Kansai Medical University, Fumizono-cho 10-15, Moriguchi, Osaka, Japan

关键词: Human T-cell leukemia virus type 1;    Tax;    Transformation;    Nuclear factor-κB;    Interferon responsive element;    2′;    5′-Oligoadenylate synthetase;    HTLV-1;    human T-cell leukemia virus type 1;    ATL;    adult T-cell leukemia;    RT-PCR;    reverse transcriptase polymerase chain reaction;    NF-κB;    nuclear factor-κB;    2;    5-OAS;    2′;    5′-oligoadenylate synthetase;    ISG;    interferon-stimulated genes;    ISRE;    interferon stimulation response element;    PBMC;    peripheral blood mononuclear cell;   

DOI  :  10.1016/S0014-5793(03)00200-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Gene expression in Rat-1 fibroblast cells transformed by Tax from human T-cell leukemia virus type 1 was studied using the reverse transcriptase polymerase chain reaction differential display technique. The analysis revealed eight genes that were upregulated and one gene that was suppressed in Tax-transformed cells. Interestingly, at least four of the upregulated genes were interferon-stimulated genes. Promoter analysis of the 2′,5′-oligoadenylate synthetase gene, which was activated in both Tax-transformed Rat-1 cells and primary adult T-cell leukemia cells, demonstrated that Tax indirectly activates its interferon-responsive enhancer element in a nuclear factor-κB pathway-dependent manner, indicating a close association of interferon signaling with the transformation by Tax.

【 授权许可】

Unknown